Data Supports Spiroplasma

Lines of evidence supporting the role of an extreme thermo-acidophilic bacterium (spiroplasma sp.) in the pathogenesis of chronic wasting disease and the other transmissible spongiform encephalopathies.


MORPHOLOGICAL
  • SPIROPLASMA IDENTIFIED BY TEM IN CJD BRAIN TISSUES
  • SPIROPLASMA IN AQUEOUS FLUID OF SHEEP WITH TERMINAL SCRAPIE
  • EXPERIMENTALLY SPIROPLASMA-INFECTED ANIMAL MODEL
RAT MODEL 
  • SPIROPLASMA INDUCES SPONGIFORM ENCEPHALOPATHY
DEER MODEL
  • CLINICAL EVIDENCE OF BRAIN STEM LESIONS
  • MORPHOLOGICAL EVIDENCE OF BRAIN STEM LESION
RUMINANT MODEL- SHEEP AND GOATS
  • MORPHOLOGICAL EVIDENCE OF BRAIN STEM LESION
  • LOCALIZATION TO EYES- RETINOPATHY
MOLECULAR DETECTION OF ORGANISM IN TSE-AFFECTED TISSUES (PCR)
  • CULTURE OF SPIROPLASMA FROM CWD & OTHER TSE-AFFECTED TISSUES
  • PASSAGE IN EMBRYONATED EGGS
GROWTH IN CELL-FREE BRUCELLA SPECIAL MEDIA
  • GROWTH ON AGAR WITH FORMATION OF SUBSURFACE PLAQUES
  • IDENTICAL BIOLOGICAL CHARACTERISTICS OF TRANSMISSIBLE TSE AGENT AS SHOWN BY ENUMERATION OF SUBSURFACE PLACUES ON AGAR
IMMUNOLOGICAL
  • REACTION OF SCRAPIE HYPERIMMUNE SERA WITH SPIROPLASMA FIBRILS
  • ELISA REACTION OF SPIROPLASMA RECOMBINANT HSP60 WITH CJD SERA
OTHER
  • FORMATION OF ALPHA SYNUCLEIN IN SPIROPLASMA MIRUM INFECTED MAMMALIAN TISSUE CULTURE
  • NORMAL PRION PROTEIN ISOFORM ON CELL SURFACE IS RECEPTOR FOR BACTERIUM-EXPLAINS INTERACTION OF PRION WITH SPIROPLASMA


 

Welcome to the TSE Research Center

The PURPOSE of this website is to present information on transmissible spongiform encephalopathies (TSE) in a clear manner and to provide e...